Studies towards the development of potential antibiotics from the leaves and stem bark extracts of Pterocarpus Osun

Abstract

Pterocarpus osun is a leguminous tree that belongs to the family Fabaceae. P. osun is an ingredient used in traditional therapy for the treatment of sickle cell disorder, diarrhea, dysentery, and management of freshly severed umbilical cord to prevent infections. The goal of this study was to look at some of these old assertions. Hexane, ethyl acetate, and methanol were used to extract the leaf and stem bark of P.osun. The antioxidant capacity of the extracts was determined using the 2, 2-Diphenyl-1- PicrylHydrazyl DPPH scavenging technique. Standard procedures were used to screen the extracts for secondary metabolites and antibacterial activity. The DPPH scavenging activities of all the fractions compared favorably well with the ascorbic acid used as standard. Highest activity (of 85.0%) for the extracts was recorded at 0.0625mg/mL for the methanol stem bark extract and the least activity (62.7%) for ethyl acetate leaf extract at a concentration of 1.0mg/ml. Terpenes, cardiac glycoside, saponins, steroids, alkaloids, tannins, and flavonoids are some of the phytochemicals detected in the extracts. Four of the tested organisms were sensitive to all the extracts while four others were resistant. The inhibitory zones were measured between 18mm and 29mm. For the crude and crude fractions, the extracts were evaluated for their minimum inhibitory concentration (MIC) and minimal bacteria/fungicidal concentrations (MBC/MFC) at 2.5mg/mL and 0.5mg/mL, respectively. The findings of these investigations suggest that the plant could be used as a supplemental medicine source. In future medication development projects, more chemical analysis for chemical ingredients is required.